Composite

Part:BBa_K1867001:Design

Designed by: Shien Yang Lee   Group: iGEM15_NYU-AD   (2015-09-16)


pro_LldD_term


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene was placed under control of a strong constitutive promoter and terminator to encourage high levels of expression to maximise amount of L-lactate secreted.


Source

Coding sequence from Ecocyc database, accessed at: http://biocyc.org/ECOLI/sequence?object=EG11963 The coding sequence was synthesized as a Biobrick compatible part, which was then assembled to attach a promoter+rbs upstream and a terminator downstream. Promoter+rbs and terminator were registry parts BBa_K608002 and BBa_B0015 respectively.

References